Polar, Normal-Phase & HILIC Columns
Dedicated Selectivity for Polar Compounds and Normal-Phase Workflows
ANPEL Polar, Normal-Phase, and HILIC Columns provide a comprehensive selection of stationary phases designed for the retention and separation of polar analytes, carbohydrates, and compounds not adequately retained by conventional reversed-phase chromatography. The category encompasses unbonded silica, cyano, diol, amino, and dedicated HILIC chemistries.
Depending on the selected stationary phase, these columns support normal-phase separations using non-polar organic mobile phases, or Hydrophilic Interaction Liquid Chromatography (HILIC) workflows using highly organic, aqueous-organic mobile phases. This versatility allows laboratories to address challenging separations across pharmaceutical, food, clinical, and chemical-research applications.
Explore the Polar and HILIC Range
Choose the Right Column for Your Method
Select the stationary phase based on the target analytes and the intended chromatographic mode. For conventional normal-phase separations using solvents like hexane or heptane, unbonded Silica, CN, Diol, or NH2 columns are appropriate choices. Diol and CN phases often provide faster equilibration and alternative selectivity compared with bare silica.
For highly polar, hydrophilic compounds, HILIC mode is generally preferred. Dedicated HILIC columns or HILIC-Amide phases provide strong retention for polar species using mobile phases with high organic content (typically acetonitrile) and a small proportion of aqueous buffer. NH2 columns are frequently selected for sugar and carbohydrate analysis in HILIC mode.
Select the Appropriate Column Format
Once the stationary phase is selected, choose the column dimensions and particle size that match the required resolution and system capabilities. Smaller particle sizes (such as 3 μm) and core-shell formats can provide higher efficiency for complex separations, while standard 5 μm particles offer robust performance for routine analysis. Internal diameter and column length should be matched to the sample load, flow rate, and analysis-time requirements.
Typical Applications
ANPEL Polar, Normal-Phase, and HILIC Columns are used for the analysis of carbohydrates, sugars, polar pharmaceuticals, metabolites, water-soluble vitamins, structural isomers, and compounds lacking sufficient retention on C18 columns. Refer to the specific product series page to confirm the recommended applications and operating modes for each stationary phase.
Important Selection Note
Stationary phases such as CN and NH2 can often be used in either normal-phase or reversed-phase/HILIC modes; however, switching a single column between immiscible mobile-phase systems is generally not recommended. Always verify the column’s pH range, solvent compatibility, pressure limits, and current SKU availability before use. Confirm product-specific specifications on the relevant series page and against the latest technical documentation.
