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Liquid-liquid extraction(LLE) tubes

Liquid-liquid extraction (LLE) tube

CNW macroporous diatomite tube
Description Packing Use Packaging
What’s SLE, and what’s the difference between SLE and LLE/SPE

SLE: solid supported liquid-liquid extraction: is a method to use high capacity solid packings as supporter to achieve higher extraction efficiency. After packings absorbe water-soluble samples, analytes spread on the water layer of packings' surface.And then add proper water insoluble organic solution, there will form a boundary which has specific surface area, to achive maximum extraction efficiency.


The products are always used in clinical chemistry and toxicology, and used for pretreatment of urine, whole blood, plasma, serum, gastric juice, amniotic fluid, feces, and animal/plant tissue. And also used in environment and residual analysis, such as industrial water, drinking water, and waste water analysis. And for acid and alkali compounds,such as drug and drug metabolism in body fluid.

The advantage compared with traditional LLE(liquid-liquid extraction):

● Simplify extraction method
● Prevents emulsification
● Reduced glassware requirements
● Use less sample processing time
● Greater reproducibility
● More suitable for automated,enlarge treatment capacity

The difference between SPE(Solid Phase Extraction)and SLE:

SLE has excellent extraction effect for high concentration samples, while SPE is more suitable for the concentration of low concentration samples.
Physical characteristics and parameter of packing:

The support of SLE is diatomaceous earth with high pore volume, which undergo high temperature purification, and have high water sucking capacity.

Loading capacity comparison table

How to use CNW macroporous diatomite tube
1. Water sample preparation: Add sodium chloride to accelerate two-phase distribution,use buffer to adjust the ionic state of analytes (low pH to extract acidic compounds, high pH to extract basic compounds)
2. Choose suitable tube according to maximum treatment capacity
3. Load sampel into extraction tube, just rely on gravity
4. Wait for 3-10min, let packing absorbe water sample sufficiently
5. Elute by organic solvent immiscible with water, if it is mixed solvent, you should consider the content of watersoluble solvent to make sure it is immiscible with water
6. Collect concentrated eluent or detect directly

Common elute solvent:
dichloromethane    chloroform    tert butyl ether    acetic ether
diethyl ether      n-hexane      cyclohexane        methybenzene
chloroform -methanol(90:10,v/v)(85:15,v/v)    diethyl ether -ethanol(90:10,v/v)(80:20,v/v)
dichloromethane –isopropanol (90:10,v/v) (85:15,v/v)