Edge tool for Lutein, Vitamin E testing – Athena C30 HPLC column

Lutein and Vitamin E are nutrition enhancers commonly used in food; National Standard GB14880-2012 (Nutrition Enhancer use standard in food) clearly stipulates dosage of these two substance. In 2017, GB5009.248 – 2016 (Determination of lutein in food) and GB5009.82—2016 (Determination of vitamin A, D, E in food) respectively stipulate the analysis method of lutein and vitamin E, and make clearly requirement to separation of isomer.     

According to the method, ANPEL using Athena C30 column respectively test lutein and vitamin A, E in food; each ingredient has a perfect peak pattern, a good separation and a high stability, totally meet the testing requirement of national standard.


Determination of lutein (GB5009.248 – 2016)
Analysis condition
Column: Athena C30, 4.6 x 250mm, 5μm (8.462552.0001)
Flow rate: 1.0mL/min
Temperature: 30℃
Detection: UV, 455nm
Injection Volume: 50μL
Mobile phase:  A: Methyl alcohol : Water (88 : 12, volume ratio, include 0.1% BHT)
              B: TBME (include 0.1% BHT)
Gradient condition:


Spectrogram
standard substance:


Sample:


standard curve



Determination of vitamin A and E (GB5009.82—2016)
Analysis condition
Column: Athena C30, 4.6 x 250mm, 5μm (8.462552.0001)
Flow rate: 0.8mL/min
Mobile phase:  A: Methyl alcohol  B: Water
Temperature: 20℃
Detection: UV, Vitamin A 325nm; Vitamin E 294nm
Gradient condition:

Spectrogram