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CASE
Study of application of 60Co－γ irradiation in degradation of four Sudan red dyes and two veterinary drugs residues in foods
Key words: γ－radition; chloramphenicol; 3－methyl－quinoxaline－2－carboxylic acid; Sudan dyes; degradation
Details
Abstract: The effects of radiation dose on the degradation rate of 3 － methyl － quinoxaline － 2 － carboxylic acid ( MQCA) ，chloramphenicol and SudanⅠ～Ⅳ were studied.60Co－γ ray at different radiation doses( 0～10 kGy) wasapplied for the foods of animal origin contaminated with residues.The results showed that 60Co－γ irradiation could significantly degrade MQCA in chicken and SudanⅠ～Ⅳ in egg liquid.When the radiation dose was 9 kGy，the degradation rates were up to 99.0% and 83.5%～95.7%，respectively.The degrading effect of sudanⅠ～Ⅳin egg powder were not obvious，the degradation rates were only 9.3%～22.3%.And the degradation of chloramphenicol was 75.3%.To all of them，the degradation rate went up with the increase of radiation dose.This study showed that irradiation treatment was effective to degrade some compounds in foods.
1. Materials and methods
1.1 materials and instruments
Natural pollution samples: acetonitrile, glacial acetic acid, ethyl acetate, acetone All formic acids were chromatographically pure from Merck company of Germany Anhydrous sodium sulfate is analytically pure, and the chemical reagent of Sinopharm group is limited Company; experimental water: ultra pure water; sbeq-ca4954 Sudan red only SPE column Shanghai Anpu Scientific Instrument Co., Ltd Oasis Max anion exchange column (mqca detection) USA Waters.
Triplequad5500 triple quadrupole liquid chromatography mass spectrometry was used Ab SCIEX, & waters; equipped with ultra performance liquid chromatograph (USA Waters company); cr22g high speed frozen centrifuge HIMAC Hitachi Multi function nitrogen blowing instrument hn200 anon Haineng Instrument Co., Ltd Line electron accelerator Tongfang Weishi is075, 7.5 MeV, 5 kW.
1.2 experimental methods
1.2.1 for the preparation of natural pollution samples, 20 0.8 kg eels were choose After the administration of chloramphenicol to fish, the muscle tissues were taken The product was made into freeze-dried powder and vacuum packed to obtain chloramphenicol 20 laying hens (about 1.0 kg) were fed with SudanThe feed of red Ⅰ, Sudan Ⅱ, Sudan Ⅲ and Sudan Ⅳ was obtained The eggs were homogenized to prepare naturally contaminated positive Sudan dye egg solution Part of the whole egg liquid was made into lyophilized powder, and the moisture content was reduced by irradiation Solution of the influence of vacuum packaging, get natural pollution Sudan dye 20 healthy white mice aged 30 days (about 1.0 kg) were choose Feather chickens were fed with feed containing olaquindox and slaughtered after appropriate withdrawal period The chicken was homogenized and vacuum packed to obtain samples of naturally contaminated olaquindox (containing The metabolite mqca of olaquindox was found. All the samples were cooled at - 18 ℃ Cryopreservation.
1.2.2 irradiation treatment three kinds of samples were irradiated with 60Co - γ The voltage is 7.5 MeV, the current is 0.41 Ma and the power is 3 kW. Egg powder The irradiation doses of the other samples were 0, 0 and 9 kGy, respectively 1. 2,3,4,5,6,7,8,9,10 kGy, and then frozen at - 18 ℃
deposit.
1.2.3 detection methods all the tested objects in this experiment refer to the country Standards or industry standards for extraction, purification, testing, detection methods such as
Table 1.
1. Materials and methods
1.1 materials and instruments
Natural pollution samples: acetonitrile, glacial acetic acid, ethyl acetate, acetone All formic acids were chromatographically pure from Merck company of Germany Anhydrous sodium sulfate is analytically pure, and the chemical reagent of Sinopharm group is limited Company; experimental water: ultra pure water; sbeq-ca4954 Sudan red only SPE column Shanghai Anpu Scientific Instrument Co., Ltd Oasis Max anion exchange column (mqca detection) USA Waters.
Triplequad5500 triple quadrupole liquid chromatography mass spectrometry was used Ab SCIEX, & waters; equipped with ultra performance liquid chromatograph (USA Waters company); cr22g high speed frozen centrifuge HIMAC Hitachi Multi function nitrogen blowing instrument hn200 anon Haineng Instrument Co., Ltd Line electron accelerator Tongfang Weishi is075, 7.5 MeV, 5 kW.
1.2 experimental methods
1.2.1 for the preparation of natural pollution samples, 20 0.8 kg eels were choose After the administration of chloramphenicol to fish, the muscle tissues were taken The product was made into freeze-dried powder and vacuum packed to obtain chloramphenicol 20 laying hens (about 1.0 kg) were fed with SudanThe feed of red Ⅰ, Sudan Ⅱ, Sudan Ⅲ and Sudan Ⅳ was obtained The eggs were homogenized to prepare naturally contaminated positive Sudan dye egg solution Part of the whole egg liquid was made into lyophilized powder, and the moisture content was reduced by irradiation Solution of the influence of vacuum packaging, get natural pollution Sudan dye 20 healthy white mice aged 30 days (about 1.0 kg) were choose Feather chickens were fed with feed containing olaquindox and slaughtered after appropriate withdrawal period The chicken was homogenized and vacuum packed to obtain samples of naturally contaminated olaquindox (containing The metabolite mqca of olaquindox was found. All the samples were cooled at - 18 ℃ Cryopreservation.
1.2.2 irradiation treatment three kinds of samples were irradiated with 60Co - γ The voltage is 7.5 MeV, the current is 0.41 Ma and the power is 3 kW. Egg powder The irradiation doses of the other samples were 0, 0 and 9 kGy, respectively 1. 2,3,4,5,6,7,8,9,10 kGy, and then frozen at - 18 ℃
deposit.
1.2.3 detection methods all the tested objects in this experiment refer to the country Standards or industry standards for extraction, purification, testing, detection methods such as
Table 1.
