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CASE
Determination of indicator polychlorinated biphenyls in Spirulina by solid-phase extraction and gas chromatography
KEY WORDS: gas chromatography; indicator polychlorinated biphenyls; Spirulina
Details
ABSTRACT: Objective To establish a method for the determination of trace polychlorinate biphenyls in Spirulina by gas chromatography, so as to investigate the residues of 7 kinds of indicator polychlorinated biphenyls (PCBs) in Spirulina. Methods After extracted with acetonitrile, the sample extract was purified by Florisil column.Under the chromatographic conditions, the analysis was carried out with a capillary column (DB-17, 30 m×0.25 mm,0.25 μm) at a flow rate of 1.0 mL/min, and the sample volume was 1.00 μL. Monitoring with an electron-capture detector, all the target analytes were separated by temperature-programming of the column. Results Good linearities were obtained in the range of 10~50 μg/L for 7 kinds of indicator PCBs with the correlation coefficients greater than 0.999 by external method. The average recoveries at three spiked levels of 25, 35 and 45 μg/kg of the 7
kinds of indicator PCBs in Spirulina ranged from 90.8% to 112% with the RSDs of 0.526%~3.89%. Conclusions This established method is simple, rapid and accurate, and can be used for the routine analysis for the indicator PCBsin Spirulina.
kinds of indicator PCBs in Spirulina ranged from 90.8% to 112% with the RSDs of 0.526%~3.89%. Conclusions This established method is simple, rapid and accurate, and can be used for the routine analysis for the indicator PCBsin Spirulina.
